CTLA4 blockade and GM-CSF combination immunotherapy alters the intratumor balance of effector and regulatory T cells
J. Clin. Invest. Sergio A. Quezada, et al. 116:1935 doi:10.1172/JCI27745 [Go to this article.]

Figure 1
CTLA4 blockade enhances Teff proliferation in vitro. CD4⁺CD25⁺ Tregs and CD4⁺CD25^– Teffs were isolated from lymph nodes and stimulated in vitro with irradiated T cell–depleted splenocytes in the presence of 10 μg/ml anti-CD3 in the presence or absence of 50 μg/ml anti-CTLA4 monoclonal antibody. [³H]thymidine was added for the last 8 hours of a 72-hour culture. The data are representative of 3 independent experiments.