JCI - Longitudinal noninvasive PET-based β cell mass estimates in a spontaneous diabetes rat model

Longitudinal noninvasive PET-based β cell mass estimates in a spontaneous diabetes rat model

Fabiola Souza, Norman Simpson, Anthony Raffo, Chitra Saxena, Antonella Maffei, Mark Hardy, Michael Kilbourn, Robin Goland, Rudolph Leibel, J. John Mann, Ronald Van Heertum, Paul E. Harris

Published in Volume 116, Issue 6

J Clin Invest. 2006; 116(6):1506–1513 doi:10.1172/JCI27645

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Figure 1

Representative reconstructed serial PET images of coronal abdominal planes of BB-DP rats during progression of autoimmune diabetes.

The bodies of the pancreata of animals A and G are identified by large arrows; the livers are identified by the letter L. Approximately 300 μCi of (+)-α-[¹¹C]DTBZ was used for imaging. Euglycemic male rats were imaged to establish a baseline at approximately 7 weeks of age; these animals were reimaged 2 or 3 times during the following 5 weeks. Whole-venous-blood glucose concentrations of 7-week-old animals fasted for 4 hours ranged from 90 to 135 mg/dl. Reconstructed images represent the summed data of the entire 1-hour-long scanning period. Display ranges of images are not equivalent. Quantitation of activity within pancreas and liver regions of interest during the scan period is shown in the time-activity curves in the panels at the right. The age of the animal in weeks appears in the lower right corner. The average activity of [¹¹C]DTBZ in nCi/cc of tissue within the liver or pancreatic regions of interest versus the duration of the PET scan in seconds is plotted on the ordinates and abscissae, respectively. Solid lines represent activity in the liver, and open circles represent activity in pancreas regions of interest.