CXCR2 ligands and G-CSF mediate PKCα-induced intraepidermal inflammation
J. Clin. Invest. Christophe Cataisson, et al. 116:2757 doi:10.1172/JCI27514 [
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Figure 5Blocking G-CSF prevents neutrophilia in K5-PKCα mice. (
A) A single dose of TPA (1 μg) in acetone was applied to the shaved backs of K5-PKCα transgenic mice and WT littermates. Blood was drawn at various times, and ELISA for G-CSF was performed on sera. Bars represent the mean ± SEM for 6 animals, and results are representative of at least 2 independent experiments. *
P < 0.05 versus the respective control. (
B) Agarose gel stained with ethidium bromide showing RT-PCR product for G-CSF and actin. RNA was extracted from K5-PKCα skin punch biopsy samples 3 hours after TPA or acetone treatment. Each lane represents an individual mouse. Peripheral blood neutrophil counts (
C) and skin MPO activity (
D) 6 hours after a single dose of TPA (1 μg) in acetone was applied to the shaved backs of K5-PKCα mice that had been injected intraperitoneally with either control IgG or G-CSF–neutralizing antibodies. Bars represent the mean ± SEM for 8 animals, and results are representative of 2 independent experiments. **
P < 0.0001 versus TPA-treated IgG group. NS, not significant versus TPA-treated IgG group.
