CXCR2 ligands and G-CSF mediate PKCα-induced intraepidermal inflammation
J. Clin. Invest. Christophe Cataisson, et al. 116:2757 doi:10.1172/JCI27514 [
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Figure 3K5-PKCα primary keratinocytes produce MIP-2 and KC through PKCα-mediated NF-κB activation. Left panels: Culture supernatants from K5-PKCα primary keratinocytes were collected at 1, 3, and 6 hours after TPA treatment. KC and MIP-2 concentrations were determined by ELISA. Bars represent the mean ± SEM of triplicate determinations. Results are representative of 3 independent experiments. *
P < 0.05 versus time 0. Right panels: KC and MIP-2 concentrations in culture supernatant collected from K5-PKCα keratinocytes transduced with A-CMV (control) or degradation-resistant IκBα (IκBαSR) adenovirus after a 3-hour TPA or DMSO treatment. Bars represent the mean ± SEM of triplicate determinations. Results are representative of 3 independent experiments. *
P < 0.05 versus the respective DMSO-treated/A-CMV control.
