Figure 7
Nck1 and Nck2 are present in podocytes.
(A) Lysate obtained from an immortalized podocyte cell line cultured under permissive (33°C) or nonpermissive (37°C) conditions or obtained from isolated mouse glomeruli was resolved by SDS-PAGE and immunoblotted using the indicated antibodies. (B) Mouse glomeruli isolated by sieving were extracted in buffer containing 1% Triton X-100 at 4°C. The lysate was subjected to flotation gradient centrifugation. DRM was identified in lane 5 at the interface between 5 and 30% Optiprep densities. Fractions (1 ml) were collected and analyzed by immunoblotting with the indicated antibodies.
