KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
J. Clin. Invest. Christopher H. Parsons, et al. 116:1963 doi:10.1172/JCI27249 [Go to this article.]

Figure 5
Distinct NOD/SCID spleen cell populations were latently infected with KSHV. (A) For cells from mice injected with KSHV, intranuclear LANA staining was noted in the B220⁺, Ly49⁺, CD11b⁺, and CD11c⁺ but not CD3⁺ or CD117⁺ populations. (B) Analogous populations of cells from mice receiving UV-KSHV revealed no specific staining suggestive of LANA. (C) The proportion of LANA⁺ cells within the positive populations in A. (D) Four populations (see also text and Table 1 for details) accounted for nearly all LANA⁺ cells. (E and F) BF imaging and specific nuclear staining with DRAQ5 (NUC) allowed determinations of area (E) and N/C ratios (F) for individual cells. P < 0.001 for mean size comparisons between small-sized (~115 μm²; CD3⁺ and B220⁺) and intermediate-sized (~130 μm²; Ly49⁺, CD11b⁺, and CD11c⁺) cells and between intermediate-sized and large-sized (~155 μm²; CD117⁺) cells. P < 0.001 for N/C comparisons between populations with low (~0.31; Ly49⁺ and CD11b⁺) and high (~0.33-0.35; B220⁺, CD3⁺, CD11c⁺, and CD117⁺) N/C ratios. P values were calculated as outlined in Methods. (G) Relative proportions of different cell types are similar in mice 4 months after injection with either KSHV (white bars) or UV-KSHV (black bars). SM, surface marker.