KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
J. Clin. Invest. Christopher H. Parsons, et al. 116:1963 doi:10.1172/JCI27249 [Go to this article.]

Figure 2
KSHV latent and lytic protein expression escalated over time following intravenous injection of NOD/SCID mice. NOD/SCID spleens were collected 1 month (A and B) and 4 months (C and D) after intravenous injection with PBS (A), KSHV (B and C), and UV-KSHV (D). Spleen sections were incubated with DAPI (blue) and mAbs to either LANA (A–D, large panels) or K8.1 (C and D, insets) and the images merged to assess colocalization. Solely to emphasize the qualitative aspects of the LANA staining pattern, we chose C from a mouse spleen with one of the highest overall infection rates (3%). Staining characteristic of cytoplasmic or cell-surface localization of K8.1 was also evident within rare cells (approximately 0.01%) in sections from KSHV-injected mice (C, inset, arrows) while no staining over background staining was observed within sections from UV-KSHV–injected mice (D, inset). Original magnification, ×40 (large panels); ×20 (insets).