JCI - HTLV-1 propels untransformed CD4+ lymphocytes into the cell cycle while protecting CD8+ cells from death

HTLV-1 propels untransformed CD4⁺ lymphocytes into the cell cycle while protecting CD8⁺ cells from death

David Sibon, Anne-Sophie Gabet, Marc Zandecki, Christiane Pinatel, Julien Thête, Marie-Hélène Delfau-Larue, Samira Rabaaoui, Antoine Gessain, Olivier Gout, Steven Jacobson, Franck Mortreux, Eric Wattel

Published in Volume 116, Issue 4

J Clin Invest. 2006; 116(4):974–983 doi:10.1172/JCI27198

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Figure 3

Infected CD4⁺ lymphocytes that express tax display both nuclear abnormalities and cytokinesis defects.

Clones 13 (A), 15 (C), 57 (B), 67 (F–H), and 68 (D and E) were cytospun, fixed, and stained with May-Grünwald and Giemsa. Label notation indicates the unique clone number, the phenotype, the infected or uninfected nature of the cells, and the corresponding amount of tax mRNA, measured in arbitrary units. Compared with uninfected CD8⁺ clones (A), HTLV-1–positive CD8⁺ clones (B) displayed enlarged cells with enlarged cytoplasm. Compared with uninfected CD4⁺ clones (C), HTLV-1–positive CD4⁺ clones (D and E) frequently display giant cells, some harboring enlarged nucleus (arrows). Multinucleated cells frequently displayed nuclei of heterogeneous sizes, as identified by arrowheads in E and G and shown at higher magnification in F. Chromatin bridges connecting nuclei in multinucleated cells are identified by smaller arrows in G and H. The distribution of cellular nuclearity according to phenotype and infectious status is shown in I. *P < 0.001; **P < 0.0001, infected vs. uninfected cells. Magnification, ×40 (A–E), ×100 –H).