Prelamin A and lamin A appear to be dispensable in the nuclear lamina
J. Clin. Invest. Loren G. Fong, et al. 116:743 doi:10.1172/JCI27125 [
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Figure 5Lamin C is normally targeted to the nuclear envelope in
Lmna+/+ primary embryonic fibroblasts. Wild-type and
Lmna+/+ cells were incubated with antibodies against lamin A/C (red) and LAP2 (green) or DAPI to visualize DNA (blue), and the cells were examined by epifluorescence microscopy. The red fluorescence in wild-type cells identifies both lamin A and lamin C, whereas the red fluorescence in
Lmna+/+ cells represents only lamin C staining. Both lamin C and LAP2 in
Lmna+/+ cells were located in the nucleus and nuclear rim, and their distribution was indistinguishable from that of wild-type cells.
