Prelamin A and lamin A appear to be dispensable in the nuclear lamina
J. Clin. Invest. Loren G. Fong, et al. 116:743 doi:10.1172/JCI27125 [
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Figure 4Emerin localization is normal in
Lmna+/+ primary embryonic fibroblasts. The localization of lamins A and C (red) and emerin (green) in wild-type,
Lmna –/ –, and
Lmna+/+ fibroblasts was determined by confocal fluorescence microscopy. DAPI was used to visualize DNA. To obtain side-by-side comparisons with
Lmna –/ – cells, wild-type and
Lmna+/+ fibroblasts were mixed together with
Lmna –/ – cells in equal proportions and plated on the same coverslip. (
A–
C) Wild-type cells plus
Lmna –/ – cells. Cells were stained with an anti–lamin A/C antibody, and wild-type cells (arrows) were identified by red fluorescence. As expected based on the studies of Sullivan and coworkers (
11), emerin was normally located within the nuclei of wild-type cells, but was mislocalized to the ER in
Lmna –/ – cells. (
D–
F)
Lmna+/+ cells plus
Lmna –/ – cells. Cells were plated and stained as described for wild-type cells.
Lmna+/+ cells (arrows) were identified by red fluorescence. Emerin was located in the nucleus of
Lmna+/+ cells with a pattern indistinguishable from that of wild-type cells.
