Strain-dependent embryonic lethality and exaggerated vascular remodeling in heparin cofactor II–deficient mice
J. Clin. Invest. Ken-ichi Aihara, et al. 117:1514 doi:10.1172/JCI27095 [
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Figure 2Noninterference of
HCII targeting vector introduction into murine genome.
(
A) FISH analysis using a
phosphoglycerate kinase–neomycin cassette probe and genomic map around the
HCII genome. Single green spots in male and female
HCII+/– murine chromosomes indicate no random integration and adequate homologous recombination of the
HCII targeting vector. Original magnification, ×1,000; ×4,000 (insets). (
B) Real-time PCR analysis for mRNA quantification of genes located around the
HCII genome in
HCII+/+ (white bars) and
HCII+/– mice (black bars). Values were normalized by arbitrarily setting the measurement for
HCII+/+ mice to 1.0.
Ube2l3, ubiquitin-conjugating enzyme E2L3;
Pik4ca, phosphatidylinositol 4-kinase, catalytic, alpha polypeptide;
Snap29, synaptosomal-associated protein.
