JCI - Essential role of TNF family molecule LIGHT as a cytokine in the pathogenesis of hepatitis

Essential role of TNF family molecule LIGHT as a cytokine in the pathogenesis of hepatitis

Sudarshan Anand, Pu Wang, Kiyoshi Yoshimura, In-Hak Choi, Anja Hilliard, Youhai H. Chen, Chyung-Ru Wang, Richard Schulick, Andrew S. Flies, Dallas B. Flies, Gefeng Zhu, Yanhui Xu, Drew M. Pardoll, Lieping Chen, Koji Tamada

Published in Volume 116, Issue 4

J Clin Invest. 2006; 116(4):1045–1051 doi:10.1172/JCI27083

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Figure 1

Pathogenic role of upregulated LIGHT in hepatitis.

(A) BALB/c mice were injected i.v. with 25 mg/kg ConA. At the indicated time points, the mice were sacrificed, and total RNA was extracted from liver and spleen. LIGHT, HVEM, LTβR, and GAPDH expression was examined by Northern blot analysis. (B and C) Wild-type (open circles) and LIGHT-deficient (filled circles) mice were injected i.v. with 30 mg/kg ConA. The survival (B) and serum ALT levels (C) of recipient mice were monitored. Sera were collected 18 hours after ConA injection, and the ALT levels were measured as described in Methods. SF, Sigma-Frankel. *p = 0.003.