Heme oxygenase-1 is a modulator of inflammation and vaso-occlusion in transgenic sickle mice
J. Clin. Invest. John D. Belcher, et al. 116:808 doi:10.1172/JCI26857 [Go to this article.]

Figure 7
Biliverdin or CO treatment inhibits NF-κB activation in the lungs of sickle mice. S+S-Antilles mice were untreated, treated with biliverdin injections (50 μmol/kg i.p. twice, at 16 hours and 2 hours), or treated with inhaled CO (250 ppm in air for 1 hour per day for 3 days). Two hours after the second biliverdin injection or 24 hours after the third CO treatment, mice were exposed to 3 hours of hypoxia (7% O₂/93% N₂) and 2 hours of reoxygenation in room air. After 2 hours of reoxygenation, the lungs were harvested, and NF-κB activation was measured in organ homogenates by EMSA. n = 3 mice per group. Below each bar is a representative NF-κB band from the EMSA. *P < 0.05, untreated versus biliverdin or CO.