Heme oxygenase-1 is a modulator of inflammation and vaso-occlusion in transgenic sickle mice
J. Clin. Invest. John D. Belcher, et al. 116:808 doi:10.1172/JCI26857 [Go to this article.]

Figure 6
Further upregulation of HO-1 by hemin inhibits NF-κB activation and VCAM-1 and ICAM-1 overexpression in the organs of sickle mice. S+S-Antilles mice were either untreated or injected with hemin (40 μmol/kg/d, i.p.) for 3 days. Twenty-four hours after the third injection, the organs were harvested from mice in ambient air. NF-κB activation was measured by EMSA, and VCAM-1 and ICAM-1 expression was measured by Western blotting in organ homogenates of the lungs, liver, and spleen of sickle mice. (A) The NF-κB, VCAM-1, and ICAM-1 bands are shown for each organ and each sickle mouse. (B) The bar graph shows the mean band intensity (n = 3 mice per group) ± SD for each organ treatment group. *P < 0.05, untreated versus hemin.