Treg-mediated immunosuppression involves activation of the Notch-HES1 axis by membrane-bound TGF-β
J. Clin. Invest. 116:4 doi:10.1172/JCI26490 [Go to this article.]

Figure 3
High-level expression of Notch ligands on TGF-β^m+, but not TGF-β^m–, cells.

(A) CD4⁺CD25⁺ cells were purified from the spleens of naive or tolerized mice. The isolated CD4⁺CD25⁺ cells were stained for the expression of surface TGF-β1 and either Jagged-1, Delta-1, or Delta-4. No TGF-β^m+ cells were detected among the CD4⁺CD25⁺ cells isolated from naive mice, while 10% of the CD4⁺CD25⁺ cells isolated from tolerized mice expressed TGF-β on the cell surface. The dot plots shown were generated by gating on CD4⁺CD25⁺TGF-β^m+ cells from tolerized mice after staining with isotype (goat IgG) control, anti–Jagged-1, anti–Delta-1, or anti–Delta-4 antibodies. (B) TGF-β^m– T cells from tolerized animals were similarly stained and analyzed for the expression of the 3 Notch ligands. Similar numbers of gated events are shown in each panel.