Disease-associated mutations affect intracellular traffic and paracellular Mg2+ transport function of Claudin-16
J. Clin. Invest. 116:4 doi:10.1172/JCI26323 [Go to this article.]

Figure 3
Cell surface expression of Cldn16 mutants linked to FHHNC.

HeLa cells transiently expressing the indicated HA-tagged Cldn16 mutants were incubated in the presence of anti-loop antibody for 1 hour at 37°C and then immunostained to detect the HA tag (red) or the anti-loop antibody (green). Merged images are shown. Shown are representative images of 2–3 independent experiments.