The latency-associated nuclear antigen of Kaposi sarcoma–associated herpesvirus induces B cell hyperplasia and lymphoma
J. Clin. Invest. Farnaz D. Fakhari, et al. 116:735 doi:10.1172/JCI26190 [Go to this article.]

Figure 7
PCR analysis of VDJ_H and DJ_H rearrangements in tumors of LANA transgenic mice. Total spleen genomic DNAs were used in the PCR reactions analyzing tumors. (A–C) PCR analysis of total spleen genomic DNAs from 4 LANA transgenic mice with tumors, 2 C57BL/6 mice older than 300 days (WT), and 2 different murine B cell lines resolved on a 1.5% Tris-acetate-EDTA–agarose gel, used as controls for the clonality. WT lanes demonstrate VDJ_H1, VDJ_H2, VDJ_H3, and VDJ_H4 rearrangements (A and B) using V_H7183 (A) or V_HJ558 primers (B) and DJ_H1, DJ_H2, DJ_H3, and DJ_H4 rearrangements (C) using Dq52 primers. The primers were designed to amplify all rearrangement products between J_H4 and V_H7183, V_HJ558, or Dq52; 4 different bands would be expected by the combination of each primer set. M, 100-bp DNA Ladder (New England Biolabs Inc.) (D) DNA samples used for the Ig rearrangement PCRs were quantified using real-time quantitative PCR to demonstrate equal total DNA concentrations.