Blocking the α4 integrin–paxillin interaction selectively impairs mononuclear leukocyte recruitment to an inflammatory site
J. Clin. Invest. Chloé C. Féral, et al. 116:715 doi:10.1172/JCI26091 [Go to this article.]

Figure 6
The α4 integrin–paxillin interaction is not required for normal architecture and lymphocyte distribution within the spleen. (A) Histology of spleens from WT and α4(Y991A) is shown. Sections from paraffin embedded spleens were stained with H&E. Immunofluorescence of splenic sections stained with MOMA-1 (green) to detect macrophages and B220 (red) to detect B cells is also shown. The locations of macrophages (Mac), follicular B cells (FB), and marginal zone B (MZB) cells are indicated. (B) The relative percentage of T and B cells in the spleen is shown. Splenocytes from WT and α4(Y991A) mice were analyzed by flow cytometry for CD3 (T cell marker) and B220 (B cell marker). (C) The relative percentage of B cell subpopulations in the spleen is shown. Cells were stained for B220, CD21/35, and CD23, and flow cytometry was used to distinguish marginal zone B cells (CD21/35⁺CD23^–), follicular B cells (CD21/35⁺CD23⁺), and immature B cells (CD21/35^–CD23^–). (D) Follicular architecture in spleens from WT and α4(Y991A) mice. Immunofluorescence of tissue sections was performed with anti-IgM (red) and anti-IgD (green) to distinguish the position of B cell follicles with a rim of marginal zone B cells (IgM⁺IgD^–). Arrows indicate the marginal zone around the follicle. Results are representative of 6–8 different mice. Magnification, ×4 (A, top); ×20 (A, bottom, and D).