Blocking the α4 integrin–paxillin interaction selectively impairs mononuclear leukocyte recruitment to an inflammatory site
J. Clin. Invest. Chloé C. Féral, et al. 116:715 doi:10.1172/JCI26091 [Go to this article.]

Figure 3
Impaired recruitment of T and B2 cells during thioglycollate-induced peritonitis in mice with disruption of the α4 integrin–paxillin interaction. (A–D) Peritoneal cells collected as described in Figure 2 were subjected to flow cytometric analysis to distinguish total B cells (A), T cells (B), B1 cells (C), and B2 cells (D). The number of T and B cells in lavage fluid was calculated from total lymphocyte counts corrected for the relative percentage of T and B cells determined by flow cytometry staining with B220-PerCP (B cell marker) and CD3-FITC (T cell marker). B1 and B2 subpopulations were distinguished as being B220-PerCP⁺Mac-1^–APC⁺ and B220-PerCp⁺Mac-1^–APC^–, respectively. Results are mean ± SEM of 4 mice for each time point. *P < 0.05, 2-tailed Student’s t test.