Blocking the α4 integrin–paxillin interaction selectively impairs mononuclear leukocyte recruitment to an inflammatory site
J. Clin. Invest. Chloé C. Féral, et al. 116:715 doi:10.1172/JCI26091 [
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Figure 2The recruitment of mononuclear leukocytes to the peritoneum in response to thioglycollate is impaired in mice with disrupted α4 integrin-paxillin interaction. (
A–
C) WT and α4(Y991A) mice were injected intraperitoneally with thioglycollate, and peritoneal lavage fluid collected at the indicated time points. Total cell number in the lavage fluid was measured with a hemocytometer, and differential cell counts were performed on cytospin slides after modified Wright-Giemsa staining. Results are shown for total lymphocyte (
A), monocyte/macrophage (
B), and neutrophil (
C) counts. *
P = 0.013, 2-tailed Student’s
t test. Results are mean ± SEM of 4–8 mice for each time point. (
D) Ratios of adoptively transferred WT/α4(Y991A) splenic lymphocytes found in the spleen, blood, peripheral LN (PLN), mesenteric LN (MLN), and thioglycollate-induced inflamed peritoneal cavities (Periton.) of recipient WT mice. Ratios of differentially labeled cells were assessed by flow cytometry and normalized to the starting input ratio. Results are mean ± SEM of 8 mice from 3 separate experiments. **
P = 0.037, WT vs. α4(Y991A), 1-tailed Student’s
t test.