Blocking the α4 integrin–paxillin interaction selectively impairs mononuclear leukocyte recruitment to an inflammatory site
J. Clin. Invest. Chloé C. Féral, et al. 116:715 doi:10.1172/JCI26091 [Go to this article.]

Figure 2
The recruitment of mononuclear leukocytes to the peritoneum in response to thioglycollate is impaired in mice with disrupted α4 integrin-paxillin interaction. (A–C) WT and α4(Y991A) mice were injected intraperitoneally with thioglycollate, and peritoneal lavage fluid collected at the indicated time points. Total cell number in the lavage fluid was measured with a hemocytometer, and differential cell counts were performed on cytospin slides after modified Wright-Giemsa staining. Results are shown for total lymphocyte (A), monocyte/macrophage (B), and neutrophil (C) counts. *P = 0.013, 2-tailed Student’s t test. Results are mean ± SEM of 4–8 mice for each time point. (D) Ratios of adoptively transferred WT/α4(Y991A) splenic lymphocytes found in the spleen, blood, peripheral LN (PLN), mesenteric LN (MLN), and thioglycollate-induced inflamed peritoneal cavities (Periton.) of recipient WT mice. Ratios of differentially labeled cells were assessed by flow cytometry and normalized to the starting input ratio. Results are mean ± SEM of 8 mice from 3 separate experiments. **P = 0.037, WT vs. α4(Y991A), 1-tailed Student’s t test.