Figure 8
Injection of NF/PDGF-BB does not increase cell proliferation after infarction.
(A) Immunohistochemistry of BrdU was used to measure total cell proliferation at the periinfarct areas after 14 days of MI. Also shown are the percentages of BrdU-positive cells (arrows) in the different groups (n ≥ 6 in each group; in total, ~300 cells counted in each animal). Scale bars: 5 μm. (B) Immunofluorescence costaining of Ki67 with cell-specific markers was also used to examine cell proliferation 14 days after MI. Red, cell markers, including tropomyosin for cardiomyocytes, isolectin for endothelial cells, α-SMA for pericytes/VSMC, and vimentin for fibroblasts; yellow, Ki67 cells and cell markers; blue, DAPI. Arrows indicate Ki67-positive cells. Scale bars: 5 μm. Also shown are the percentages of Ki67-positive cells in total cell population and in individual endothelial and fibroblast population. (C) DNA synthesis measurement using [3H]thymidine incorporation was performed to study the mitogenic effect of 10 ng/ml PDGF-BB and 10% FBS on cultured rat cardiomyocytes and cardiac fibroblasts.
