Figure 1
Endothelial cells promote cardiomyocyte survival via PDGF-BB signaling.
Cardiomyocytes (CM), cocultured with endothelial cells (EC) or fibroblasts (FB), were treated with doxorubicin (Dox) (A) or chelerythrine (Chele) (B) to induce apoptosis. TUNEL (A) and DNA fragmentation cytometry (B) were used to quantify cardiomyocyte apoptosis with α-sarcomeric actinin staining to identify cardiomyocytes. Arrows indicate areas of DNA fragmentation. G indicates gated area for quantifying the DNA fragmentation. (C) Cardiomyocyte apoptosis, with or without endothelial coculture, was induced by doxorubicin with or without neutralizing antibodies against PDGF-BB (P-BB), PDGF-AA (P-AA), PDGFR-β, or PDGFR-α, and was determined using propidium iodide cytometry. **P < 0.001.
