Figure 5
TNF-α stimulus induced greater lung inflammation in Nrf2-deficient mice.
(A) BAL fluid analysis at 6 hours after i.p. injection of TNF-α (10 μg/mouse). (B) Histopathological analysis of lungs of Nrf2+/+ and Nrf2–/– mice by H&E staining 24 hours after i.p. injection of TNF-α (10 μg/mouse). Vehicle-treated lungs are not shown. Magnification, ×20. (C) Gene expression analysis of TNF-α, IL-1β, and IL-6 by real-time PCR in the lungs of Nrf2–/– and Nrf2+/+ mice 30 minutes after TNF-α challenge. Data are presented as mean α SEM. *Differs from vehicle control of the same genotype. †Differs from LPS-treated wild-type mice. < 0.05.
