Figure 4
Ischemia/reperfusion in isolated guinea pig hearts elicits the release of mast cell renin, Ang II formation, NE release, and reperfusion arrhythmias.
Fifty-five guinea pig hearts were subjected to 20-minute global ischemia followed by 30-minute reperfusion either in the absence (n = 15) or presence of lodoxamide (Lodox, 10 μM; n = 13), cromolyn (Crom, 300 μM; n = 11), BILA2157 (BILA, 100 nM; n = 6), or EXP3174 (EXP, 300 nM; n = 10). Bars indicate means α SEM. (A) Duration of reperfusion arrhythmias (VT/VF). (B and C) Overflow of NE and Ang I–forming activity collected over 6 minutes either before ischemia or at the start of reperfusion. *Significantly different from own control; P < 0.05 by 1-way ANOVA with Dunnett’srocedure.
