JCI - Anaphylactic shock depends on PI3K and eNOS-derived NO

Anaphylactic shock depends on PI3K and eNOS-derived NO

Anje Cauwels, Ben Janssen, Emmanuel Buys, Patrick Sips, Peter Brouckaert

Published in Volume 116, Issue 8

J Clin Invest. 2006; 116(8):2244–2251 doi:10.1172/JCI25426

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Figure 4

BSA anaphylactic shock in WT and eNOS^–/– mice.

(A) Survival plot of C57BL/6 mice sensitized with BSA, treated with vehicle (controls), l-NAME, or WM, and finally challenged with BSA in EB. The number of mice in each experimental group is shown in parentheses. **P = 0.0022. (B) Temperature plot (mean ± SD) of mice sensitized by BSA or PBS and challenged 15 days later with BSA in EB (BSA + EB) or EB alone. The total number of mice is shown in parentheses; numbers beneath time points indicate the number of mice still alive at the indicated times. (C) Survival plot of WT and eNOS^–/– mice sensitized with BSA and challenged with BSA in EB. Total number of mice is shown in parentheses. ***P = 0.0007. (D) Temperature plot (mean ± SD) of mice sensitized by BSA or PBS and challenged with BSA in EB or EB alone. The total number of mice is shown in parentheses, numbers above the time points indicate the number of mice still alive at the indicated times. (E) Western blot for p-eNOS in kidney homogenates of individual, BSA-sensitized mice 12 minutes after BSA challenge. Where indicated, mice had been pretreated with WM. (F) Vascular leakage assayed by EB extravasation into ear tissues. Inner ear rims were removed 90 minutes after i.v. challenge with BSA in EB or EB only and subsequently extracted in formamide. Plots show mean OD₆₂₀ ± SD. ^#P < 0.001; ^##P < 0.01.