Signals for stress erythropoiesis are integrated via an erythropoietin receptor–phosphotyrosine-343–Stat5 axis
J. Clin. Invest. Madhu P. Menon, et al. 116:683 doi:10.1172/JCI25227 [Go to this article.]

Figure 2
Epo expression in EpoR-HM mice is not substantially elevated during steady-state erythropoiesis but is hyperactivated in response to anemia. (A) EpoR-HM, WT-EpoR, and EpoR-H mice were treated with phenylhydrazine (PHZ, at 100 mg/kg, or PBS only). Base-line (steady-state) and phenylhydrazine-induced levels of renal Epo transcripts (at 0, 20, and 40 hours after phenylhydrazine dosing) then were determined by quantitative RT-PCR (and are normalized for coassayed levels of actin transcripts). (B) Representative primary PCR data also are shown. Tx, transcript.