Expression of AR in transgenic mice, normal human hearts, and macrophages. (A) Western blot of AR protein in aortas from Ldlr–/– and hAR-Ldlr–/– mice. Aortas were obtained from 8-week-old mice on chow diets, the aortas were harvested and homogenized, and equal amounts of protein were loaded on SDS gels. AR was detected using a polyclonal antibody that recognizes both human and mAR. (B) Northern blot analysis of heart expression of human and mAR gene. (C) Real-time PCR analysis of macrophage expression of hAR and mAR mRNA. Values are expressed as mean ± SEM; n = 5 mice per group. For human macrophages, 2 samples were assayed in duplicate 3 times.