Akt1/protein kinase Bα is critical for ischemic and VEGF-mediated angiogenesis
J. Clin. Invest. Eric Ackah, et al. 115:2119 doi:10.1172/JCI24726 [
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Figure 3Ischemia-stimulated EPC mobilization is impaired in
Akt1–/– mice; administration of
Akt1–/– EPCs does not improve perfusion of ischemic limbs. (
A) Representative photomicrographs showing Ac-Dil-LDL–positive EPCs isolated from peripheral blood mononuclear cells of WT and
Akt1–/– mice after femoral ligation. Magnification, ×200. (
B) For each animal, Ac-Dil-LDL–positive cells in 10 low-power (×100) fields were counted. EPC counts were obtained at baseline and 6 days after induction of limb ischemia;
n = 3–6. (
C) Representative laser Doppler blood flow images of the limbs of WT mice at postoperative day 14 after femoral ligation and intravenous administration of medium, WT EPCs, or
Akt1–/– EPCs (see Methods). (
D) Quantitative analysis of laser Doppler blood flow images. Data are expressed as a ratio of the left (ischemic) to right (control) limb perfusion;
n = 4. **
P < 0.01.
