Akt1/protein kinase Bα is critical for ischemic and VEGF-mediated angiogenesis
J. Clin. Invest. Eric Ackah, et al. 115:2119 doi:10.1172/JCI24726 [
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Figure 1Characterization of tissue and vascular expression of Akt1 and Akt2. (
A) RT-PCR analysis of RNA isolated from mouse lung fibroblasts using
Akt1-,
Akt2-, and
Hsp90-specific primers. (
B) Expression of Akt1 and Akt2 protein in homogenates from heart and gastrocnemius muscle by Western blot with antibodies against Akt1, Akt2, or Hsp90 as a loading control. (
C) Akt1 and Akt2 expression in various blood vessels by Western blot using antibodies against Akt1, Akt2, and β-actin as a loading control. (
D) p-Akt levels in various blood vessels from WT,
Akt1–/–, and
Akt2–/– mice. Lysates from 3 animals per group were pooled and analyzed by Western blot using p-AktS473– and p-AktT308–specific Akt antibodies. (
E) Relative expression of Akt isoforms in blood vessels. Lysates from WT mice were analyzed by SDS-PAGE and quantitative Western blot. Relative protein amounts of Akt1 and Akt2 in the vessels were quantified using standard curves obtained by running recombinant mouse Akt1 and Akt2 proteins. Data represent the mean of 2 pooled samples;
n = 3.
