JCI - Acquisition of full effector function in vitro paradoxically impairs the in vivo antitumor efficacy of adoptively transferred CD8+ T cells

Acquisition of full effector function in vitro paradoxically impairs the in vivo antitumor efficacy of adoptively transferred CD8⁺ T cells
J. Clin. Invest. 115:6 doi:10.1172/JCI24480 [Go to this article.]

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Figure 2

Cytofluorometry and Western blot analysis of effective and impaired CD8⁺ T cells. Pmel-1 CD8⁺ T cells at progressive stages of differentiation were generated using multiple in vitro stimulations with 1 μM hgp10025–33 and 30 IU/ml of rhIL-2. (A) Flow cytometry for the surface expression of IL-7Rα and KLRG-1. Results after gating for CD8⁺ cells are shown. (B) Detection of BID and its activated, truncated C-terminal cleavage products (tBID) p15 and p13 and BAD by Western blot analysis.