The role of insulin receptor substrate 2 in hypothalamic and β cell function
J. Clin. Invest. Agharul I. Choudhury, et al. 115:940 doi:10.1172/JCI24445 [
Go to this article.]
Figure 4Hypothalamic function in RIPCreIrs2KO, NesCreIrs2KO, and POMCCreIrs2KO mice. (
A) Body weight was measured in 12-week-old male mice of the indicated genotypes. (
B) Total body fat was determined by MRI in 16-week-old male mice of the indicated genotypes. (
C) Cumulative 24-hour food intake was measured in 12-week-old male mice of the indicated genotypes. (
D) Fasting blood leptin levels of 12-week-old male mice of the indicated genotypes were measured after a 16-hour overnight fast. (
E–
G) Cumulative food intake, percent reduction in food intake compared with baseline, and reduction in bodyweight were measured over a 3-day period of treatment with leptin (5 mg/kg) or vehicle in 6–8-week-old male mice of the indicated genotypes. (
H) Food intake and fasting leptin levels were determined in 5-week-old male mice of the indicated genotypes. Data in
A–
H data represent the mean ± SEM for 8–10 animals of each genotype. *P < 0.05, **P < 0.01, and ***P < 0.001.
