The role of insulin receptor substrate 2 in hypothalamic and β cell function
J. Clin. Invest. Agharul I. Choudhury, et al. 115:940 doi:10.1172/JCI24445 [Go to this article.]

Figure 2
Analysis of deletion of Irs2 in islets and hypothalami from RIPCreIrs2KO, POMCCreIrs2KO, and NesCreIrs2KO mice. (A) We performed PCR analysis to detect recombination of the Irs2 locus in DNA from islets and hypothalami (hypo) of control and knockout mice. The presence of a 1.3-kb PCR product indicates recombination and deletion of the Irs2 gene. (B and C) Western blot analysis of Irs2 in islets from control and RIPCreIrs2KO mice (B) and whole brain and hypothalamic lysates from control and NesCreIrs2KO mice (C). (D) Immunofluorescence analysis for Irs2 expression in the hypothalami of RIPCreZEG and RIPCreIrs2KOZEG mice. Colocalization of GFP (green) and Irs2 (red) is seen in RIPCreZEG mice (indicated by white arrows) and no colocalization in RIPCreIrs2KOZEG mice. RIPCreGFP, GFP expression in RIPCre cells. (E) Colocalization of POMCCre expression and Irs2 in POMCCreZEG mice. (F) Colocalization of POMCCre expression and Irs2 in POMCCrelacZ mice and no colocalization of GFP and Irs2 in POMCCreIrs2KOlacZ mice. Confocal images of representative arcuate nucleus fields are shown in D–F. Scale bars: 10 μm.