ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation
J. Clin. Invest. Istvan Boldogh, et al. 115:2169 doi:10.1172/JCI24422 [Go to this article.]

Figure 2
RWE increases ROS levels in cultured epithelial cells. (A) Effect of inhibitors of NADPH oxidase on the pollen extract–induced increase in ROS levels in A549 cells. The presence or absence of NADPH in the reaction mixture is indicated. PN, pecan tree extract; TG, timothy grass extract. ^#P < 0.001; ^##P < 0.0001. (B) Induction of intracellular ROS by RWE in various epithelial cells and its inhibition by NAC. Shown are RWE (black bars), RWE plus NAC (white bars), and X+XO (gray bars). (C) Effect of inhibitors of NADPH oxidase on the RWE-induced increase in ROS levels in NHBE cells grown in air-liquid interphase. (D) Effect of protease inhibitors on ROS production induced by HDM extracts and RWE in A549 cells. Shown are cells with (white bars) and without (black bars) protease inhibitors.