Figure 1
Effects of CEACAM1 expression on the murine endothelial cell line SVEC4-10.
(A) Two-dimensional Matrigel transwell assay with different CEACAM1-infected SVEC4-10 clones. After retrovirally mediated infections, 3 clones per CEACAM1 mutant were selected and subjected to a transmigration assay with NIH3T3 fibroblast–conditioned medium as a chemoattractant in the bottom chamber. All assays were carried out in triplicate, and the number of migrated cells was averaged. UT, untransfected SVEC4-10 cells; neo, mock-infected. (B) Three-dimensional fibrin assay with CEACAM1-infected SVEC4-10 cells. SVEC4-10 cells expressing different forms of CEACAM1-L were grown in a 3-dimensional fibrin matrix for 3 days, and endothelial cell protrusions were documented. Experiments were carried out in triplicate with 3 different clones per mutant. Representative images of 1 clone are shown. Magnification, ×200.
