Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion
J. Clin. Invest. Marie Fischer, et al. 116:2748 doi:10.1172/JCI24274 [
Go to this article.]
Figure 2CD30-Fc stimulation of CBMCs induces chemokine expression. (
A) CBMCs at a concentration of 2 × 10
5 cells/ml were stimulated with 10 μg/ml of CD30-Fc, and total RNA was prepared. Expression of different cytokine mRNAs was analyzed after 3 hours of stimulation using RNase protection assay. A reference RNA (ref RNA) supplied by the manufacturer was also included. No stim., no stimulation. (
B) CBMCs (2 × 10
5 cells/ml) were stimulated with CD30-Fc or CD6-Fc (10 μg/ml) for 3 hours, and total RNA was prepared. Expression of different chemokines was analyzed by RNase protection assay. Data presented in
A and
B are from a single representative experiment out of 3 performed. CBMCs from 3 different donors were treated with CD30-Fc, CD6-Fc (10 μg/ml, 1 × 10
5 cells/ml), or IgE cross-linking. Supernatants were recovered by centrifugation. Release of IL-8 was analyzed using ELISA after (
C) 30 minutes and (
D) 24 hours. (
E) Secretion of chemokines was analyzed after 24 hours.
