The AML1-ETO fusion gene and the FLT3 length mutation collaborate in inducing acute leukemia in mice
J. Clin. Invest. Christina Schessl, et al. 115:2159 doi:10.1172/JCI24225 [Go to this article.]

Figure 2
Analyses of CFU-S frequencies. (A) Primary BM cells retrovirally transduced with GFP, AML1-ETO, AML1-ETO-L148D, FLT3-LM, or FLT3-LM-KD vectors or with combinations of the different vectors were isolated by FACS 48 hours after infection and injected into lethally irradiated mice to assess initial (day 0) CFU-S numbers. CFU-S frequency per 1 × 10⁵ initiating BM cells was determined in 3 independent experiments. The number of analyzed mice and the P value compared with the GFP control are indicated. (B) CFU-S frequency of primary BM cells infected with GFP or with both AML1-ETO and FLT3-LM and treated with the inhibitor PKC412 for 48 hours compared with untreated controls.