Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure
J. Clin. Invest. Jianchao Xu, et al. 115:1275 doi:10.1172/JCI24066 [
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Figure 1Tissue expression of renalase. (
A) Northern blot analysis of human tissues using the MGC12474 clone as a probe. The upper band in skeletal muscle and lower bands in kidney and liver may represent alternatively spliced forms of renalase. (
B) Western blot analysis of rat tissues using a renalase polyclonal antibody. (
C–
F) Renalase expression in kidney. (
C) In situ hybridization analysis of human kidney. Left: antisense probe. Open arrow indicates the glomerulus; filled arrow indicates proximal tubules. Scale bar: 40 μm. Right: sense probe control. Magnification, ×200. (
D) Immunolocalization in human kidney. Left: anti-renalase antibody. Filled arrow indicates proximal tubules. Scale bar: 40 μm. Right: preimmune serum. Magnification, ×630. (
E) Immunofluorescence in human kidney. Left panels: anti-renalase antibody, thick arrow denotes the glomerulus; thin arrows indicate proximal tubules. Right panels: preimmune serum. Scale bars: 40 μm. (
F and
G) Renalase expression in heart. (
F) In situ hybridization analysis of human heart. Left: antisense probe. Open arrow indicates blood vessels; filled arrow indicates ventricular myocytes. Scale bar: 40 μm. Right: sense probe control. Magnification, ×200. (
G) Immunolocalization in human heart. Left: anti-renalase antibody. Open arrow indicates blood vessels; filled arrows indicate ventricular myocytes. Scale bar: 40 μm. Right: preimmune serum. Magnification, ×630.
