The matrix component biglycan
is proinflammatory and signals through
Toll-like receptors 4 and 2 in macrophages
J. Clin. Invest. Liliana Schaefer, et al. 115:2223 doi:10.1172/JCI23755 [
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Figure 4Activation of MAPKs and NF-κB by BGN in macrophages. (
A) Phosphorylation of ERK and p38 in
Bgn+/0 macrophages after 10–120 minutes of incubation with BGN (Western blots). (
B) Effects of the MEK1/2 inhibitor (U0126) on the phosphorylation of ERK in
Bgn+/0 macrophages 30 minutes after induction with BGN. (
C and
D) Reduced levels of TNF-α (
C) and MIP-2 (
D) in the presence of U0126 or SB203580 (an inhibitor of p38 MAPK) in culture media from
Bgn+/0 macrophages 6 hours after induction with BGN (4 μg/ml). Data are given as means ± SD from 3–4 animals in each group. *
P < 0.05 for macrophages with BGN and inhibitor versus macrophages with BGN and without inhibitor. (
E) Electrophoretic mobility shift assay of nuclear extracts from
Bgn+/0 macrophages cultured for 30 minutes with or without BGN. C1 to C3 represent negative controls: C1, without protein; C2 and C3, macrophages with 100 times free oligonucleotides and without (C2) or with BGN (C3).
