The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages
J. Clin. Invest. Liliana Schaefer, et al. 115:2223 doi:10.1172/JCI23755 [Go to this article.]

Figure 2
Increased expression of BGN in infiltrating cells of lung parenchyma in sepsis. (A and B) Northern blot of BGN mRNA normalized to α-tubulin (2 hours) (A) and Western blot of BGN core protein normalized to α-tubulin (8 hours) (B) from lungs of Bgn^+/0 mice after a lethal dose of LPS versus PBS. (C and D) Immunostaining of BGN (red stain) in lungs from control (C) and septic Bgn^+/0 mice (D) 8 hours after a lethal dose of LPS. (E and F) Double staining, marked by arrows, for BGN (blue) and macrophages (F4/80, red) (E) and in situ hybridization for BGN (F) in septic lungs from Bgn^+/0 mice (8 hours). The lower right inset shows a magnified view of mononuclear cells expressing BGN (white arrowhead). The upper left inset represents the sense riboprobe. (G and H) Immunostaining of macrophages (F4/80, red), with numerous F4/80–positive macrophages (lower right insets), in septic lungs from Bgn^+/0 (G) versus Bgn^–/0 mice (H). (I–L) Immunostaining of type I collagen in lungs from Bgn^+/0 (brown) (I) versus Bgn^–/0 mice (J) and Western blots of fibronectin (K) and laminin (L) normalized to β-tubulin in lungs from Bgn^+/0 versus Bgn^–/0 mice 8 hours after a lethal dose of LPS. The lower right insets in C, D, F, G, and H represent higher magnifications. Scale bars in D, H, and J apply to panels in C, G, and I, respectively.