GPR109A (PUMA-G/HM74A) mediates nicotinic acid–induced flushing
J. Clin. Invest. Zoltán Benyó, et al. 115:3634 doi:10.1172/JCI23626 [Go to this article.]

Figure 5
PUMA-G is expressed in the skin and in various immune cells. (A) Northern blot analysis of PUMA-G mRNA in the ear and brown adipose tissue (BAT) of PUMA-G^+/+ (WT) and PUMA-G^–/– (KO) animals. 18s, ribosomal RNA used as control. (B) RT-PCR of cDNAs from different tissues prepared from PUMA-G^+/+ (WT) and PUMA-G^–/– (KO) mice using PUMA-G– or GAPDH-specific primers. (C) Expression of PUMA-G in dermal MHC class II–positive cells, dendritic cells, and peritoneal macrophages. (D) Effect of NA (100 μM) and ATP (10 μM) on the [Ca²⁺]_i in macrophages prepared from wild-type or PUMA-G–deficient mice. Values on the y axis indicate the measured 340/380-nm fluorescence ratio as an indicator of the intracellular-free [Ca²⁺].