Glycyrrhizic acid alters Kaposi sarcoma–associated herpesvirus latency, triggering p53-mediated apoptosis in transformed B lymphocytes
J. Clin. Invest. Francesca Curreli, et al. 115:642 doi:10.1172/JCI23334 [Go to this article.]

Figure 4
Apoptosis signaling. (A) Analysis of mitochondrial membrane potential disruption in BJAB, BCBL-1, BC-3, and BC-1 cells untreated or treated for 4 days with 4 mM GA. In healthy cells, mitochondria appear red, as shown in untreated cells and in GA-treated BJAB cells. In dying cells with disrupted potential, the dye appears green, as shown in BCBL-1, BC-3, and BC-1 cells treated with GA. Valinomycin (100 nM) was used as positive control. (B) TUNEL assay to detect chromatin condensation in BJAB, BCBL-1, BC-3, and BC-1 cells after 4 days of treatment with 4 mM GA (green). Cells were counterstained with DAPI to localize the nuclei (blue). TPA was used as a negative control to show that lytic cycle induction does not promote chromatin condensation. Numbers indicate the percentage of TUNEL-positive cells in the same culture determined by flow cytometric analysis.