Glycyrrhizic acid alters Kaposi sarcoma–associated herpesvirus latency, triggering p53-mediated apoptosis in transformed B lymphocytes
J. Clin. Invest. Francesca Curreli, et al. 115:642 doi:10.1172/JCI23334 [Go to this article.]

Figure 3
Protein expression. (A) LANA levels of untreated and GA-treated cells. Tubulin and actin were used as controls. Lanes 1, 4, and 7: untreated cells; lanes 2, 5, and 8: cells treated with 3 mM GA; lanes 3, 6, and 9: cells treated with 4 mM GA. One representative experiment is shown. The SD from 3 independent experiments is 0.22. (B) FACS analysis of untreated and GA-treated cells stained with FITC-conjugated anti–v-cyclin. Because v-cyclin is constitutively expressed by all KSHV-infected cells, the graph shows the percentage of cells overexpressing v-cyclin. Values of GA-treated BCBL-1, BC-3, and BC-1 cells are relative to those of the same untreated cells, arbitrarily set to 1. Columns 1, 4, and 7: untreated cells; columns 2, 5, and 8: cells treated with 3 mM GA; columns 3, 6, and 9: cells treated with 4 mM GA. Values are the average of 3 experiments with 3 repeats per sample. Data represent the mean ± SEM. (C) FACS profiles of BC-3 and BC-1 cells, untreated and treated with GA and stained with FITC-conjugated anti–v-cyclin. BCBL-1 profiles were similar to those of BC-1 and BC-3 (not shown). One representative experiment is shown. (D) v-FLIP expression. Immunoblotting of cells untreated and treated with GA. Tubulin was used as control. Lane 1: BJAB cells (negative control); lanes 2, 5, and 8: untreated cells; lanes 3, 6, and 9: cells treated with 3 mM GA; lanes 4, 7, and 10: cells treated with 4 mM GA. One representative experiment is shown. (E) CDK6 expression. Immunoblotting of BC-1, BC-3, BCBL-1, BJAB, primary human lymphocytes (H-Lymph), primary human hepatocytes (H-Liver), and 293 cells. Lanes 1, 4, and 7: untreated cells; lanes 2, 5, and 8: cells treated with 3 mM GA; lanes 3, 6, and 9: cells treated with 4 mM GA. Tubulin was used as control. One representative experiment is shown. The SD from 3 independent experiments is ± 0.20.