Glycyrrhizic acid alters Kaposi sarcoma–associated herpesvirus latency, triggering p53-mediated apoptosis in transformed B lymphocytes
J. Clin. Invest. Francesca Curreli, et al. 115:642 doi:10.1172/JCI23334 [Go to this article.]

Figure 2
Latent transcript levels and luciferase assay. (A–I) Northern blot analysis. mRNA was extracted from BCBL-1 (A, D, and G), BC-3 (B, E, and H), and BC-1 (C, F, and I) cells untreated or treated with 3 or 4 mM GA. Samples were loaded in duplicate. RNA was hybridized with ORF73 probe (A–C) to detect LT1 transcripts and with ORF72 (v-cyclin) probe (D–F) to detect LT1 and LT2 transcripts. β-actin probe was used as a control (G–I). (J) Luciferase assay. First bar indicates the levels of luciferase activity in BJAB cells transfected with the LT1/LT2 promoter cloned in pGL.3-Basic (control) without GA treatment. Second and third bars indicate the levels of luciferase in BJAB cells transfected with the LT1/LT2 promoter cloned in pGL.3-Basic and then treated with 3 or 4 mM GA. Fourth bar indicates the levels of luciferase in BJAB cells transfected with only the vector pGL.3-Basic (background). The y axis indicates the fold increase relative to the background levels. Values are the averages of 3 experiments with 3 repeats per sample. Data represent the mean ± SEM. Ctrl, control.