JCI - Systemic lupus erythematosus serum IgG increases CREM binding to the IL-2 promoter and suppresses IL-2 production through CaMKIV

Systemic lupus erythematosus serum IgG increases CREM binding to the IL-2 promoter and suppresses IL-2 production through CaMKIV

Yuang-Taung Juang, Ying Wang, Elena E. Solomou, Yansong Li, Christian Mawrin, Klaus Tenbrock, Vasileios C. Kyttaris, George C. Tsokos

Published in Volume 115, Issue 4

J Clin Invest. 2005; 115(4):996–1005 doi:10.1172/JCI22854

Abstract | Full text | PDF

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Figure 2

Increased binding of CREM to the –180 site of the IL-2 promoter in SLE T cells. (A) Nuclear proteins were incubated with a ³²P-labeled oligonucleotide encoding the –180 site of the IL-2 promoter as described in Methods. Shown are 3 independently conducted EMSAs, each containing 1 pair of simultaneously purified normal (N) and SLE T cells. (B) Nuclear proteins from an SLE patient were incubated with the –180 oligonucleotide with or without antibody against CREB, CREM, or c-Jun for 15 minutes, followed by shift analysis. (C) Nuclear proteins from primary T cells were incubated with ³²P-labeled oligonucleotide encoding the –180 site of the IL-2 promoter. Where indicated, unlabeled wild-type or mutant oligonucleotides containing 2 mutated nucleotides were also added into the reaction.