Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation
J. Clin. Invest. Saori Nishio, et al. 115:910 doi:10.1172/JCI22850 [Go to this article.]

Figure 6
Apoptosis of cyst epithelial cells. (A) Apoptosis of cyst epithelial cells in the kidney of a P8 Pkd1^–/–/LZ⁺ mouse was detected by the TUNEL assay. The same section was stained with β-gal and counterstained with Nuclear Fast Red. Arrowheads indicate TUNEL-positive cells. Original magnifications, ×400. (B) Summary of results shown in A. Each graph represents the percentage of TUNEL-positive cells in LZ⁺ (black bars) and in LZ^– (Pkd1^–/–) cyst epithelial cells (white bars), respectively. The mean and SD are from 9 independent mice. *P < 0.05. (C and D) Electron microscopic analysis of the cyst epithelium of a Pkd1^–/–/LZ⁺ kidney. (C) Occasional apoptotic cells (black arrowheads) are overlaid by neighboring cells. (D) Flat cells (white arrowheads) overlay several degenerated cells that are detached from the tubular basement membrane (black arrowheads). Original magnification, ×1,500.