T cell–dependent production of IFN-γ by NK cells in response to influenza A virus
J. Clin. Invest. Xiao-Song He, et al. 114:1812 doi:10.1172/JCI22797 [Go to this article.]

Figure 7
IFN-γ and IL-2 production by NK cells and T cells in response to fluA. PBMCs from adult donors were incubated with fluA or SPG (negative control) for 12 hours, with brefeldin A added during the last 5 hours (A and B) or were incubated with fluA for 4–12 hours, with brefeldin A added for the last 4 hours (C). The cells were stained for CD56, fixed and permeabilized, and then stained intracellularly for CD3, IFN-γ, and IL-2. (A) Dot plots for a representative donor (no. 7) displaying cells gated on CD3^–CD56⁺ NK cell population (left panels) or CD3⁺ T cell population (right panels). Numbers in the dot plots refer to the percentage of cytokine-producing cells in each quadrant. (B) Summary of the levels of IFN-γ and IL-2 production by T cells from 4 donors (nos. 7–10). IL-2 was not detected in NK cells from any of these 4 donors. (C) Kinetics of IL-2 production by T cells and IFN-γ production by NK cells (donor no. 20).