T cell–dependent production of IFN-γ by NK cells in response to influenza A virus
J. Clin. Invest. Xiao-Song He, et al. 114:1812 doi:10.1172/JCI22797 [Go to this article.]

Figure 4
Depletion of CD3⁺ cells from PBMCs reduces to background levels the IFN-γ response of the NK cells to fluA. CD3⁺ cells were depleted from the PBMCs of 8 donors (PBMC-CD3). PBMCs and CD3-deleted PBMCs were incubated with fluA for 17 hours, followed by intracellular staining for IFN-γ. Displayed in the bar graphs are frequencies of IFN-γ⁺ cells in the CD56^bright perforin^– (left) and CD56^dimperforin⁺ (right) NK cell subsets. Results of 3 donors (nos. 4–6) are presented in this figure, while those of other donors are presented in Figures 5 and 9 as part of other experiments.