T cell–dependent production of IFN-γ by NK cells in response to influenza A virus
J. Clin. Invest. Xiao-Song He, et al. 114:1812 doi:10.1172/JCI22797 [Go to this article.]

Figure 2
FluA-induced IFN-γ production by the CD3^–CD56^bright perforin^– NK cell subset (labeled as CD56^bright) and CD3^–CD56^dim perforin⁺ NK cell subset (labeled as CD56^dim) in 25 donors. PBMCs were incubated with fluA for 17 hours, followed by cytokine flow-cytometric analysis (see Methods). Lines connect pairs of observations from the same donor. Black bars mark the positions of groups’ means, which were compared using paired Student’s t tests. The attained significance levels (P values) are reported. For A and C, the t tests were performed on logarithmic-transformed data. (A) The percentage of IFN-γ⁺ cells in the 2 NK cell subsets. (B) The IFN-γ MFI of IFN-γ⁺ cells in the 2 NK cell subsets. (C) The total numbers of IFN-γ⁺ cells detected simultaneously in the 2 NK cell subsets from the same PBMC aliquot of each donor.