PPARα inhibits vascular smooth muscle cell proliferation underlying intimal hyperplasia by inducing the tumor suppressor p16^INK4a
J. Clin. Invest. Florence Gizard, et al. 115:3228 doi:10.1172/JCI22756 [Go to this article.]

Figure 1
PPARα inhibits SMC G₁/S progression. (A and B) G₁-arrested hSMCs (A) and PPARα^–/– or PPARα^+/+ Sv/129 mSMCs (B) resumed growth at T0 in normal GM. The indicated concentrations of GW7647, Wy14,643 (10 μM), or vehicle (Me₂SO) were added in the normal GM. Cells were harvested at the indicated times for measure of DNA content (expressed as arbitrary units). Values are the mean ± SEM of triplicate points from a single experiment (n = 3/time point), which was repeated with 3 different cell preparations of primary SMCs with similar results. *P ≤ 0.05; ***P ≤ 0.001 vs. vehicle-treated hSMCs (A). Values in B followed by different symbols are statistically significantly different from each other. (C) Western blot analysis of in-cell pRB phosphorylation was performed using an anti-pRB antibody recognizing all species of the Rb gene product on protein extracts (30 μg) from Ad-GFP or Ad-PPARα–infected hSMCs and treated with vehicle or GW7647 (600 nM) for 10 hours.