Can antibodies with specificity for soluble antigens mimic the therapeutic effects of intravenous IgG in the treatment of autoimmune disease?
J. Clin. Invest. Vinayakumar Siragam, et al. 115:155 doi:10.1172/JCI22753 [Go to this article.]

Figure 3
Antibody reactive with soluble OVA or OVA-rbcs inhibits RES function. (A) RES blockade using soluble OVA. CD1 mice were untreated (open circles), treated intraperitoneally with IVIg (open squares), or intravenously with OVA + anti-OVA (open triangles) or OVA + control IgG (filled triangles), followed 24 hours later by intravenous injection of fluorescently labeled, TER-119–opsonized syngeneic rbcs. Blood samples were taken at the times indicated on the x axis and the percentage of fluorescent rbcs in the circulation assessed by flow cytometry. (B) RES blockade using OVA-rbcs. Mice were not pretreated (open circles), pretreated intraperitoneally with 50 mg IVIg (open squares), or pretreated intravenously with 10⁸ OVA-rbcs sensitized with 10 μg anti-OVA (open triangles) or OVA-rbcs pretreated with control IgG (filled triangles), followed 24 hours later by intravenous injection of fluorescently labeled TER-119–opsonized syngeneic rbcs. The percentage of fluorescent rbcs was assessed as in A. The percentage of fluorescent rbcs at 3 min was considered to be 100%; n = 5 mice for each group from 5 independent experiments. **P < 0.01, ^#P < 0.001. Data are presented as mean ± SEM.